Percentage change in the subsequent GABA-induced currents due to a 1-min application of 0.5 mM pCMBS− in the presence of the indicated reagent2-a
| Saturating GABA2-a | 100 nM Diazepam | 100 nM β-CCE | 100 nM Flumazenil | |
|---|---|---|---|---|
| WT2-b | −12 ± 3 (8) | 5 ± 5 (4) | 5 ± 3 (3) | 4 ± 4 (3) |
| F296C2-b | −23 ± 52-150 (10) | 24 ± 22-150 (8) | −5 ± 8 (3) | 5 ± 2 (3) |
| F298C2-b | 30 ± 62-150 (9) | 36 ± 122-150 (6) | 45 ± 112-c 2-150 (3) | 20 ± 62-150 (6) |
| L301C2-b | 37 ± 92-150 (8) | 40 ± 102-150 (5) | 3 ± 3 (3) | 52 ± 132-150 (5) |
| WT2-d | −4 ± 2 (8) | −2 ± 3 (3) | 3 ± 6 (3) | 1 ± 4 (3) |
| A300C2-d | 45 ± 62-150 (11) | 4 ± 8 (3) | 4 ± 9 (3) | 10 ± 6 (3) |
| E303C2-d | −32 ± 42-150 (20) | 5 ± 9 (4) | 12 ± 3 (3) | 3 ± 1 (3) |
Data given as means ± S.E.M. (n).
↵2-a Results in the presence of saturating GABA taken from Williams and Akabas (1999).
↵2-b Effect on EC50 GABA test pulses.
↵2-c For F298C, the effect was 9 ± 3% in the presence of 10 nM β-CCE.
↵2-d Effect on near-saturating GABA test pulses.
↵2-150 Statistically different than corresponding wild type by one-way ANOVA.