Cytotoxicity (96-h exposure) of various agents against the BE vector control, the two DT-diaphorase transfected cells, BE2 and BE5, and HT29
| Cell Line | 96-h IC50 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Streptonigrin | EO9 | Mitomycin C | EO2 | EO7 | AZQ | MeDZQ | RH1 | Tirapazamine | Menadione | |
| μM | ||||||||||
| BE vector control | 7.9 ± 1.9 | 0.5 ± 0.09 | 2.2 ± 0.3 | 1.0 ± 0.08 | 25.3 ± 10.1 | 2.2 ± 0.9 | 0.2 ± 0.03 | 0.02 ± 0.001 | 42.3 ± 14.6 | 20.7 ± 2.5 |
| BE2 | 0.06 ± 0.02 (131.7) | 0.03 ± 0.005 (16.7) | 0.3 ± 0.1 (7.3) | 0.5 ± 0.1 (2.0) | 5.0 ± 0.6 (5.1) | 4.3 ± 0.7 (0.5) | 0.2 ± 0.07 (1.0) | 0.005 ± 0.003 (4.0) | 50.1 ± 14.5 (0.8) | 22.3 ± 0.6 (0.9) |
| BE5 | 0.07 ± 0.02 (112.9) | 0.02 ± 0.008 (25.0) | 0.4 ± 0.1 (5.5) | 0.3 ± 0.02 (3.3) | 3.0 ± 0.5 (8.4) | ND | 0.2 ± 0.02 (1.0) | ND | ND | ND |
| HT29 | 0.04 ± 0.01 (197.5) | 0.03 ± 0.006 (16.7) | 0.1 ± 0.02 (22.0) | 0.3 ± 0.05 (3.3) | 7.1 ± 0.6 (3.6) | 0.6 ± 0.3 (3.7) | 0.02 ± 0.002 (10) | 0.0005 ± 0.0002 (40.0) | 32.2 ± 7.3 (1.3) | ND |
Values are mean ± SD (n = 5–8). Numbers in parentheses represent fold difference in sensitivity compared with the BE vector control line.
ND, not determined.