A1 agonist concentration-response parameters for A1receptor-stimulated [3H] inositol phosphate accumulation in high expressing CHOA1H cells transfected with the human adenosine A1 receptor
| CHOA1H | Agonist | Log EC50 | Maximum Stimulation (CPA = 100) |
|---|---|---|---|
| M | % | ||
| Control | CPA | −6.74 ± 0.09 (9) | 100 |
| NECA | −5.99 ± 0.05 (5) | 155.2 ± 5.3 | |
| R-PIA | −6.63 ± 0.27 (4) | 131.2 ± 10.1 | |
| +PTX | CPA | −6.27 ± 0.13 (6) | 100 |
| NECA | −5.46 ± 0.07 (5) | 289.6 ± 22.4 | |
| R-PIA | −5.77 ± 0.10 (4) | 129.3 ± 7.7 | |
| + ATP | CPA | −7.34 ± 0.09 (6) | 100 |
| NECA | −6.79 ± 0.09 (5) | 106.2 ± 7.2 | |
| R-PIA | −6.91 ± 0.13 (4) | 94.1 ± 6.7 | |
| + ATP/PTX | CPA | −6.43 ± 0.11 (3) | 100 |
| NECA | −5.82 ± 0.04 (5) | 149.2 ± 7.2 | |
| R-PIA | −6.05 ± 0.09 (4) | 111.0 ± 4.8 |
Values represent means ± S.E.M. The number of experiments is given in parentheses. Data were obtained under control conditions or after 24-h treatment with PTX (100 ng/ml). Where appropriate (+ATP), 0.1 mM ATP was included in all incubations.