Affinity of NECA for the wt and mutant human A2B receptors
| DNA construct | KiNECA | n |
|---|---|---|
| μM | ||
| wt human A2B | 2.2 ± 0.6 | 3 |
| A12T human A2B | 5.3 ± 2.1 | 3 |
| N273Y human A2B | 3.8 ± 1.5 | 3 |
| Nontransfected | 1 | |
| pcDNA3 | 1 |
K i values were determined in [3H]DPCPX binding experiments to transiently transfected COS-7 cells as described under Experimental Procedures. No entry forK i NECA indicates the absence of specific [3H]DPCPX binding. Data are shown as means ± S.E.M.