Table 1

Binding of [3H]quisqualate to mGlu5 and mutants in HEK cell membranes

Binding Relative to wt ± S.D. (n = 4)
fl, wt1-a 1.00 (= 910 fmol/mg protein)
fl, C(57)S0.00  ± 0.01
fl, C(93)S1.51  ± 0.25
fl, C(99)S0.00  ± 0.01
fl, C(129)S1.23  ± 0.20
fl, C(57, 93, 99, 129)S0.11  ± 0.15
t-wt1.00 (= 948 fmol/mg protein)
tC(57)S0.00  ± 0.01
tC(93)S1.75  ± 0.05
tC(99)S0.03  ± 0.03
tC(129)S2.25  ± 0.63
tC(57, 93, 99, 129)S0.18  ± 0.14
  • 1-a  fl = full-length; t = truncated.