Reconstitution Conditions | Testosterone 6β-OH | |
---|---|---|
CYP3A4 | CYP3A43 | |
nmol/min/nmol | ||
HEPES buffer (pH 7.6)1-a | ||
1:2:01-b plus 0.02% Na cholate, 15 mM MgCl2, 0.1 mM EDTA | 14.8 | 0.2 |
1:2:2 plus 0.02% Na cholate, 15 mM MgCl2, 0.1 mM EDTA | 44.4 | 0.3 |
KPO4 buffer (pH 7.4)1-c | ||
1:4:0 plus 0.04% CHAPS | 13.2 | 0.2 |
1:4:1 plus 0.04% CHAPS | 10.2 | 0.3 |
1:4:2 plus 0.04% CHAPS | 10.6 | 0.3 |
In the assays listed, the concentration of testosterone was 200 μM and a 1:1:1 mix of DOPC/DLPC/phosphatidylserine was included. The values reported represent the average of two assays performed in duplicate.