Table 1

Kinetic parameters for the transport of DNP-SG and E₂17βG by wild-type Mrp2, K325M, and R586L

	E₂17βG		DNP-SG
	K_m	IC₅₀	K_m	IC₅₀
	μM		μM
Wild-type Mrp2	3.91 ± 0.93	13.3 ± 2.3^1-163	80.8 ± 7.0	19.3 ± 3.5^1-163 ^1-154
K325M	0.40 ± 0.05^1-160	N.D.	N.D.	360 ± 89^1-160
R586L	1.40 ± 0.46^1-160	2.30 ± 1.17^1-160	308 ± 51^1-160	59.5 ± 9.8^1-163

K _m and IC₅₀ values were calculated on the basis of the data shown in Figs. 4 and 5, respectively. Each represents the obtained parameter ± computer calculated S.D. Comparison ofK _m values for E₂17βG and DNP-SG, respectively, was performed by ANOVA followed by Dunnett's test, whereas that of IC₅₀ and K _m values for E₂17βG and DNP-SG, respectively, was performed by Student'st test.

N.D., not determined.
↵1-160 p < 0.01, significantly different from wild-type Mrp2.
↵1-163 p < 0.01 and
↵1-154 p < 0.001, significantly different from respective K _m value.