COS-7 transfection | K_{d} | B_{max} |
---|---|---|
nM | fmol/mg | |
pCEFL | 19 ± 5 | 1.5 ± 0.1 (3) |
pCEFL-HA-H2r | 21 ± 6 | 11.2 ± 1.1 (3)^{*} |
pCEFL-H2r | 22 ± 4 | 12.4 ± 1.5 (3)^{*} |
The K _{d} and B _{max} values were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The K _{d} and B _{max}values of the pCEFL and the pCEFL-HAH2r and pCEFL-H2r were compared using an unpaired t test and found to be significantly different (^{*} p < 0.01).