Binding of [3H]tiotidine in COS-7 cells
| COS-7 transfection | Kd | Bmax |
|---|---|---|
| nM | fmol/mg | |
| pCEFL | 19 ± 5 | 1.5 ± 0.1 (3) |
| pCEFL-HA-H2r | 21 ± 6 | 11.2 ± 1.1 (3)* |
| pCEFL-H2r | 22 ± 4 | 12.4 ± 1.5 (3)* |
The K d and B max values were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The K d and B maxvalues of the pCEFL and the pCEFL-HAH2r and pCEFL-H2r were compared using an unpaired t test and found to be significantly different (* p < 0.01).