Half-times of dissociation of [3H]NMS from mutant and wild-type receptors
| Receptor construct | Dissociationt½ ([3H]NMS) |
|---|---|
| min | |
| M2 | 4.3 ± 0.07 |
| CR3 | 125 ± 3 |
| CR4 | 57 ± 2 |
| CR5 | 64 ± 2 |
| CR6 | 3.8 ± 0.1 |
| M5 | 58 ± 4 |
| M2 423 Thr→His | 14 ± 0.5 |
| M5 478 His→Thr | 46 ± 0.8 |
| CR6 Thr→His | 11 ± 0.8 |
| CR3 His→Thr | 57 ± 1.8 |
| M4 | 46 ± 2.1 |
| M4 436Ser→Thr | 23 ± 0.4 |
| M4 436 Ser→His | 94 ± 2.8 |
Values are the means ± S.E. in the absence of allosteric modulator, derived from three or more independent experiments. The point mutations studied in M2, M4, M5, CR3 and CR6 were all located at the position homologous to M2 423.