Table 2

Density of [¹²⁵I]α-bungarotoxin and [¹²⁵I]epibatidine binding, and α4, α5, α6, α7, β2, and β4 mRNA expression in various regions of wild-type (⁺/⁺), and heterozygous (⁺/T) mouse brains

Label	Wild-Type
	Cortex	Hippocampus		DG	Caudate Putamen	Thalamus	Medial Habenula	IPn	Substantia Nigra	Superior Colliculus
	Cortex	CA1	CA2	DG	Caudate Putamen	Thalamus	Medial Habenula	IPn	Substantia Nigra	Superior Colliculus
	%
[¹²⁵I]α-BTX
⁺/⁺	100 ± 7.2	100 ± 6.2		100 ± 8.9	100 ± 7.5					100 ± 4.2
⁺/T	56 ± 4.6^2-a	54.7 ± 2.8^2-a		55.2 ± 4.9^2-a	32.9 ± 3.3^2-a					65.7 ± 3.7^2-a
[¹²⁵I]Epi
⁺/⁺	100 ± 6.0				100 ± 2.5	100 ± 4.6	100 ± 2.7	100 ± 5.6	100 ± 6.1	100 ± 4.3
⁺/T	99.0 ± 6.5				96.9 ± 7.4	99.6 ± 2.3	99.1 ± 2.7	95.9 ± 1.6	107.5 ± 5.9	106.7 ± 1.4
α4 mRNA
⁺/⁺	100 ± 3.9	100 ± 5.6	100 ± 9.2		100 ± 7.7	100 ± 4.9	100 ± 0.8		100 ± 0.9	100 ± 3.5
⁺/−	102 ± 5.9	113 ± 9.2	98 ± 12.5		109 ± 17.4	103 ± 3.0	91 ± 3.5		95 ± 3.8	94 ± 7.3
α5 mRNA
⁺/⁺	100 ± 6.2	100 ± 7.0					100 ± 6.4	100 ± 6.8	100 ± 6.3
⁺/T	119.7 ± 8.7	98.7 ± 2.4					86.6 ± 11.5	102.4 ± 5.3	102.6 ± 5.9
α6 mRNA
⁺/⁺									100 ± 1.3	100 ± 5.7
⁺/T									103 ± 4.5	98 ± 9.7
α7 mRNA
⁺/⁺	100 ± 6.8	100 ± 3.8	100 ± 2.4	100 ± 4.3	100 ± 9.5					100 ± 6.6
⁺/T	97 ± 8.4	101 ± 3.7	104 ± 3.7	109 ± 5.9	109 ± 11.4					97 ± 8.7
β2 mRNA
⁺/⁺	100 ± 5.4	100 ± 10.0	100 ± 7.5	100 ± 4.4		100 ± 2.5	100 ± 2.1		100 ± 3.0	100 ± 2.8
⁺/T	82.2 ± 8.4	99.2 ± 13.8	98.1 ± 8.7	100.7 ± 5.3		82.5 ± 8.3	87.0 ± 6.6		88.2 ± 7.3	82.8 ± 10.5
β4 mRNA
⁺/⁺							100 ± 2.1
⁺/T							94 ± 3.5

Data represent the mean ± S.E.M. for three to five animals and are presented as a percentage of wild-type controls.

↵2-a P < 0.01; significantly different from wild-type (+/+) by Newman-Keuls test.