Table 1

Oligonucleotide primer sequences used to produce mutants

α4(C226F)
 S 5′ GCC TGC TCA TCT CCT TCC TCA CCG TGC 3′
 AS 5′ GCA CGG TGA GGA AGG AGA TGA GCA GGC 3′
α4(L258V)
 S 5′ CTT CCT GCT GGT GAT CAC CGA GAT CAT CCC 3′
 AS 5′ TCT CGG TGA TCA CCA GCA GGA AGA CGG 3′
α4(I262T)
 S 5′ CAT CAC CGA GAC CAT CCC GTC C 3′
 AS 5′ GGA CGG GAT GGT CTC GGT GAT G 3′
β2(I224T)(S226L)
 S 5′ CCT GTG TGC TCA CCA CCT TAC TAG CCA TCC 3′
 AS 5′ GGATGG CTA GTA AGG TGG TGA GCA CAC AGG 3′
β2(V254F)
 S 5′ GGC GCT CAC GTT CTT CCT GCT GC 3′
 AS 5′ AGC AGG AAG AAC GTG AGC GCC 3′
α3(V258L)
 S 5′ CGG TGT TTC TCC TGC TCA TCA CTG AGA CC 3′
 AS 5′ TCT CAG TGA TGA GCA GGA GAA ACA CCG 3′
α3(T262I)
 S 5′ GAT CAC TGA GAT CAT CCC TTC CAC C 3′
 AS 5′ TGG AAG GGA TGA TCT CAG TGA TCA CC 3′
  • S, sense; AS, antisense.