Table 2

RNA primers and probes

wt-CYP3A5mRNA
 Forward primer 5′ gggTCTCTggAAATTTgACACAgAg 3′
 Reverse primer 5′ CTgTTCTgATCACgTCgggATCT 3′
 Probe 5′ 6FAM-ATgTggggAACgTATgAAggTCAACTCCCT-TAMRA 3′
SV1-CYP3A5 mRNA
 Forward primer 5′ TCTCTggAAATTTgACACAgAgTgC 3′
 Reverse primer 5′ CAgCAAgAgTCTCACACAggAgC 3′
 Probe 5′ 6FAM-AATgTggggTATCTCTTCCCTgTTTggACCA-TAMRA 3′
CYP3A4 mRNA
 Forward primer 5′ CACAgATCCCCCTgAAATTAAgCTTA 3′
 Reverse primer 5′ AAAATTCAggCTCCACTTACggTg 3′
 Probe 5′ 6FAM-AggACTTCTTCAACCAgAAAAACCCgTTgTTCT-TAMRA 3′
CYP3A5 mRNA
 Forward primer 5′ ACAgATCCCCTTgAAATTAgACACg 3′
 Reverse primer 5′ CTTAgggTTCCATCTCTTgAATCCA 3′
 Probe 5′ 6FAM-AAggACTTCTTCAACCAgAAAAACCCATTgTTCTA-TAMRA 3′
GAPDH mRNA
 Forward primer 5′ TCCTgCACCACCAACTgCTT 3′
 Reverse primer 5′ gAggggCCATCCACAgTCTT 3′
 Probe 5′ 6FAM-CACTCATgACCACAgTCCATgCCATCAC-TAMRA 3′