RNA primers and probes
| wt-CYP3A5mRNA | |
| Forward primer | 5′ gggTCTCTggAAATTTgACACAgAg 3′ |
| Reverse primer | 5′ CTgTTCTgATCACgTCgggATCT 3′ |
| Probe | 5′ 6FAM-ATgTggggAACgTATgAAggTCAACTCCCT-TAMRA 3′ |
| SV1-CYP3A5 mRNA | |
| Forward primer | 5′ TCTCTggAAATTTgACACAgAgTgC 3′ |
| Reverse primer | 5′ CAgCAAgAgTCTCACACAggAgC 3′ |
| Probe | 5′ 6FAM-AATgTggggTATCTCTTCCCTgTTTggACCA-TAMRA 3′ |
| CYP3A4 mRNA | |
| Forward primer | 5′ CACAgATCCCCCTgAAATTAAgCTTA 3′ |
| Reverse primer | 5′ AAAATTCAggCTCCACTTACggTg 3′ |
| Probe | 5′ 6FAM-AggACTTCTTCAACCAgAAAAACCCgTTgTTCT-TAMRA 3′ |
| CYP3A5 mRNA | |
| Forward primer | 5′ ACAgATCCCCTTgAAATTAgACACg 3′ |
| Reverse primer | 5′ CTTAgggTTCCATCTCTTgAATCCA 3′ |
| Probe | 5′ 6FAM-AAggACTTCTTCAACCAgAAAAACCCATTgTTCTA-TAMRA 3′ |
| GAPDH mRNA | |
| Forward primer | 5′ TCCTgCACCACCAACTgCTT 3′ |
| Reverse primer | 5′ gAggggCCATCCACAgTCTT 3′ |
| Probe | 5′ 6FAM-CACTCATgACCACAgTCCATgCCATCAC-TAMRA 3′ |