cAMP response in GRK2-antisense clones
| Amthamine | Forskolin | |||
|---|---|---|---|---|
| EC50 | Rmax | EC50 | Rmax | |
| nM | pmol/106cells | mM | pmol/106cells | |
| U-937 | 465 ± 95 (5) | 184 ± 35 (5) | 161 ± 42 (3) | 120 ± 27 (3) |
| D2 | 562 ± 97 (3) | 143 ± 26 (3) | N.D. | N.D. |
| A2 | 426 ± 47 (3) | 281 ± 67 (3)1-150 | N.D. | N.D. |
| D5 | 472 ± 67 (3) | 292 ± 38 (3)1-150 | 121 ± 37 (3) | 116 ± 22 (3) |
| H3 | 584 ± 78 (3) | 142 ± 27 (3) | N.D. | N.D. |
EC50 and maximal response (R max) were calculated from the data in Fig. 3 using the equation for dose-response curves. The table shows the mean ± S.E.M.; the number of determinations (n) is in parentheses. TheR max of amthamine for the different clones with respect to U-937 were compared using an unpaired t test, and D5 and A2 clones were found to be significantly different.
N.D., not determined.
↵1-150 , p < 0.01.