Inhibitor | Concentration | Uptake |
---|---|---|
μM | % of control | |
Control | 100 | |
LTC4 | 1 | 40.4 ± 2.1 |
DNP-SG | 10 | 46.4 ± 6.6 |
Methotrexate | 100 | 74.0 ± 9.5 |
cAMP | 100 | 95.0 ± 0.9 |
Sulfinpyrazone | 30 | 39.5 ± 6.9 |
MK571 | 30 | 42.2 ± 7.9 |
Probenecid | 100 | 88.8 ± 10 |
Cyclosporin A | 10 | 49.6 ± 5.6 |
PSC833 | 10 | 95.4 ± 9.5 |
Verapamil | 100 | 62.3 ± 11 |
Sildenafil | 100 | 41.0 ± 3.7 |
Trequinsin | 100 | 44.3 ± 8.6 |
Zaprinast | 100 | 48.3 ± 7.5 |
17-βΕstradiol | 100 | 60.7 ± 5.9 |
16α,17β-Estriol 16-(β-d-glucuronide) | 100 | 53.0 ± 6.4 |
E23SO417βG | 100 | 45.1 ± 10. |
16α,17β-Estriol 3-(β-d-glucuronide) | 100 | 58.5 ± 13 |
17β-Estradiol 3-(β-d-glucuronide) | 100 | 60.1 ± 9.7 |
17α-Ethynyl-17β-estradiol | 100 | 51.4 ± 10 |
16α,17β-Estriol 3-sulfate | 100 | 49.4 ± 6.4 |
Glucuronic acid | 1000 | 98.7 ± 4.7 |
Taurocholate | 100 | 44.5 ± 9.8 |
Glycocholate | 100 | 58.4 ± 5.7 |
Glycolithocholate-3-sulfate | 30 | 41.8 ± 5.9 |
Vincristine | 30 | 18.4 ± 4.2 |
Paclitaxel | 30 | 22.1 ± 5.4 |
Etoposide | 30 | 49.5 ± 8.0 |
Doxorubicin | 30 | 18.2 ± 6.5 |
Cisplatin | 30 | 98.7 ± 8.0 |
ATP-dependent uptake was calculated by subtracting values obtained in the presence of 4 mM MgATP from those in the presence of 4 mM MgAMP. Transport is expressed as percent of uptake in the absence of inhibitor. Values shown are means ± S.E. of at least three measurements performed in duplicate. The concentrations of cyclosporin A and PSC833 were limited by their solubilities.