Measurement of extracellular UDP-glucose and ATP on resting cells
| UDP-Glucose | ATP | |
|---|---|---|
| nM | ||
| COS-7 | 22 ± 4 | 10 ± 3 |
| Calu-3 | 16 ± 4 | 4 ± 1 |
| CF bronchial | 14 ± 3 | 3 ± 2 |
| Normal bronchial | 12 ± 5 | 2 ± 1 |
| 1321N1 | 10 ± 4 | 3 ± 1 |
| CHO-K1 | 6 ± 3 | 5 ± 1 |
| C6 | 1 ± 1 | 1 ± 0.5 |
Cells were incubated for 2 h in 0.3 ml of DMEM and UDP-glucose and ATP accumulation in the extracellular medium were quantified as indicated under Materials and Methods. The data represent the mean value (± S.D.) from at least three experiments with duplicate samples.