Cell-cycle distribution of MCF-7 cells treated with E2, TCDD, and TCDD plus E2, and small inhibitory RNA for AhR
| MCF-7 cells were growth arrested and treated with Me2SO, TCDD, E2, and E2 plus TCDD in combination with transfected iscrambled and iAhR duplexes, and FACS analysis was determined on the cells as described under Materials and Methods. Results of duplicate analysis are presented. Values in parentheses are percentages. | |||
|---|---|---|---|
| Treatment | Cell-Cycle Phases | iScrambled | iAhR |
| Me2SO | G0/G1 | 76.7 (76.4) | 71.6 (71.9) |
| G2/M | 9.9 (10.1) | 12.2 (11.4) | |
| S | 13.5 (13.4) | 16.2 (16.7) | |
| TCDD | G0/G1 | 78.0 (78.6) | 70.2 (71.8) |
| G2/M | 10.7 (10.5) | 10.8 (12.1) | |
| S | 11.3 (10.9) | 19.0 (16.2) | |
| E2 | G0/G1 | 73.1 (71.1) | 63.7 (65.2) |
| G2/M | 7.3 (8.9) | 11.2 (9.2) | |
| S | 19.6 (20.0) | 25.1 (25.6) | |
| E2 + TCDD | G0/G1 | 74.0 (74.3) | 65.2 (64.7) |
| G2/M | 10.1 (9.6) | 10.0 (9.8) | |
| S | 15.8 (16.0) | 24.8 (25.0) | |