TABLE 1

Parameters characterizing the interactions of allosteric agents with the equilibrium binding of [³H]dimethyl-W84 and with the equilibrium binding and dissociation of [³H]NMS from M₂ receptors

The data are mean values ± S.E.M. of 3 to 22 experiments carried out in triplicate. For details, see text.

	[³H]Dimethyl-W84		[³H]NMS
Allosteric Agent	logK_i,alloster	n_H	logK_X,alloster	α	log(α × K_X,alloster)	Measured logK_X,diss	n_H	logK_i,alloster - logK_X,alloster
Dimethyl-W84	8.47±0.04^a	0.97±0.15	8.55±0.05	0.27±0.01	7.98	7.96±0.07	0.96±0.16	-0.08
W84	7.59±0.05	1.08±0.16	7.50±0.10	0.50±0.02	7.20	7.43±0.04	0.95±0.10	0.09
Gallamine	7.63±0.07	0.84±0.11	7.84±0.02	0.04±0.01	6.47	6.25±0.05	0.81±0.08	-0.21
WDuo3	6.91±0.04	1.06±0.05	6.60±0.18	1.5±0.1	6.78	7.29±0.11	0.92±0.25	0.31
Alcuronium	6.80±0.01	0.87±0.18	7.45±0.20	1.7±0.1	7.69	7.96±0.04	1.17±0.13	-0.65
Strychnine	5.05±0.22	0.78±0.12	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.

N.D., not determined
↵ a Value is the log affinity constant, logK_L, derived from homologous inhibition experiments