TABLE 3

Carboxylesterase activity and IC50 values for CPT-11 with Saos-2 cells after adenoviral transduction with AdCMVrCE and treatment with reserpine

Only one CE activity is indicated per cell line because growth-inhibition studies were performed with duplicate aliquots of the same batch of cells

Cell Line Reserpine CE Activity CPT-11 IC50 Fold change in IC50
μmol/min/mg μM
pcDNA#2 1854.9 ± 41.8 0.8
ABCG2#4 1766.3 ± 139.8 134.9 168
pcDNA#2 + 1.2
ABCG2#4 + 86.4 72