Activation | Steady-State Inactivation | ||||
---|---|---|---|---|---|
V0.5 | Slope (s) | V0.5 | Slope (s) | n | |
mV | mV | mV | mV | ||
I424C | -33.7 ± 2.8 | 4.8 ± 1.2 | -84.4 ± 2.6 | 6.1 ± 0.6 | 5 |
I425C | -30.1 ± 1.6* | 6.9 ± 0.2 | -86.5 ± 1.4 | 6.3 ± 0.6 | 6 |
F426C | -35.4 ± 1.3 | 4.1 ± 0.3 | -97.7 ± 2.3* | 7.3 ± 0.3 | 5 |
L427C | -39.0 ± 2.1 | 4.9 ± 0.8 | -86.7 ± 2.9 | 5.5 ± 0.1 | 7 |
G428C | -34.9 ± 1.1 | 6.3 ± 0.5 | -87.2 ± 1.2 | 5.4 ± 0.3 | 5 |
S429C | -35.4 ± 2.7 | 4.9 ± 0.4 | -88.2 ± 2.1 | 6.7 ± 0.5 | 5 |
F430C | -37.1 ± 2.5 | 5.1 ± 0.5 | -90.6 ± 1.2 | 6.0 ± 0.4 | 5 |
Y431C | -33.2 ± 1.4 | 5.2 ± 0.3 | -81.2 ± 1.9* | 5.9 ± 0.3 | 9 |
L432C | -31.0 ± 1.5 | 7.0 ± 0.7 | -86.7 ± 1.5 | 5.7 ± 0.2 | 5 |
I433C | -32.6 ± 1.6 | 6.3 ± 0.4 | -86.3 ± 1.3 | 5.9 ± 0.2 | 5 |
N434C | -31.2 ± 2.6 | 5.9 ± 1.0 | -103 ± 1.8* | 7.2 ± 0.8 | 8 |
L435C | -34.6 ± 2.4 | 4.6 ± 0.4 | -91.3 ± 2.8 | 7.3 ± 0.8 | 4 |
I436C | -25.7 ± 2.5* | 5.7 ± 0.5 | -85.8 ± 1.2 | 6.4 ± 0.8 | 6 |
II-S6 | |||||
V779C | -36.8 ± 2.5 | 4.7 ± 0.1 | -91.6 ± 2.6 | 6.3 ± 0.1 | 8 |
M780C | -30.4 ± 1.2 | 4.2 ± 0.2 | -87.3 ± 2.4 | 6.4 ± 0.4 | 5 |
V781C | -34.5 ± 3.8 | 4.9 ± 0.4 | -87.6 ± 1.3 | 7.1 ± 0.6 | 8 |
I782C | -39.3 ± 2.8 | 5.3 ± 0.3 | -79.9 ± 1.2* | 5.1 ± 0.4 | 8 |
G783C | -32.9 ± 2.6 | 5.0 ± 0.2 | -88.9 ± 3.1 | 6.5 ± 0.3 | 6 |
N784C | -36.4 ± 1.8 | 4.2 ± 1.8 | -88.7 ± 0.9 | 6.8 ± 0.3 | 5 |
L785C | -24.1 ± 1.3* | 4.9 ± 0.2 | -87.1 ± 2.0 | 7.1 ± 0.3 | 8 |
V786C | -41.7 ± 1.2 | 4.3 ± 0.7 | -81.8 ± 1.4* | 4.6 ± 0.2 | 5 |
V787C | -32.7 ± 2.1 | 6.4 ± 0.9 | -90.9 ± 3.0 | 5.8 ± 0.7 | 5 |
L788C | -34.7 ± 2.7 | 4.8 ± 0.5 | -87.2 ± 1.1 | 5.8 ± 0.3 | 5 |
N789C | -29.1 ± 1.5* | 4.9 ± 0.2 | -85.7 ± 1.5 | 5.9 ± 0.8 | 4 |
L790C | -30.3 ± 1.1 | 5.3 ± 0.9 | -84.1 ± 1.6 | 5.5 ± 0.3 | 5 |
F791C | -33.4 ± 0.6 | 5.1 ± 0.8 | -85.3 ± 1.3 | 5.3 ± 0.3 | 4 |
↵* P < 0.05 compared with wild-type (WT).