TABLE 2

DFB potentiation of human and rat mGluR5 activation by glutamate, DHPG, and quisqualate

Maximum potentiation is the ratio of the maximum to the minimum response determined by nonlinear curve fitting of mean data (see Fig. 3). Data are expressed as mean ± S.E.M. (n)

[Agonist] EC50 for Potentiation Maximum Potentiation
μM -fold
Human mGluR5a CHO cells
    300 nM Glutamate 2.6 ± 0.4 (6) 3.1
    1 μM DHPG 2.0 ± 0.5 (6) 3.4
    8 nM Quisqualate 2.3 ± 1.2 (6) 3.7
Rat mGluR5 CHO cells
    300 nM Glutamate 5.3 ± 1.4 (5) 4.1
    1 μM DHPG 3.6 ± 1.4 (5) 6.2
    8 nM Quisqualate 3.6 ± 1.1 (4) 3.7