TABLE 3

Effect of DFB on FLIPR agonist concentration-response curves: human and rat mGluR5

EC50 change at 100 μM DFB was calculated from the EC50 values determined by nonlinear curve fitting of the mean data (see Fig. 4) Data are expressed as mean ± S.E.M. (n)

Agonist Control Agonist EC50 EC50 Change at 100 μM DFB
-fold
Human mGluR5a CHO cells
    Glutamate 642 ± 30 nM (5) 2.2
    DHPG 2.36 ± 0.17 μM (5) 2.3
    Quisqualate 21.4 ± 0.7 nM (5) 2.2
Rat mGluR5 CHO cells
    Glutamate 659 ± 48 nM (5) 1.9
    DHPG 2.07 ± 0.12 μM (5) 2.0
    Quisqualate 22.0 ± 0.9 nM (5) 1.7