TABLE 2

Effect of cisplatin and 17-AAG combination on the cell cycle distribution in colon cancer cell lines Results shown reflect percentage of cells in each phase of cell cycle according to DNA content analysis by FACS. Cells were treated for 24 h with 30 μM DDP and 500 nM 17-AAG alone or in combination before FACS analysis. Data represent average values from two independent experiments.

Cell line Control DDP 17-AAG DDP+17-AAG
G1 S G2 G1 S G2 G1 S G2 G1 S G2
HCT 116 59 24 17 39 59 2 85 0 15 50 26 24
DLD1 53 32 15 48 50 2 39 1 60 38 42 20
HT-29 79 11 10 60 40 0 49 1 50 65 24 11
SW480 68 26 6 64 35 1 59 2 39 60 32 8