Gene profiling in response to activation of Frizzled chimera: QPCR validation of targets identified by DNA microarray analysis Genes of interest regulated by activation of either Frizzled-1 or Frizzled-2 chimeras were first identified by gene profiling were subjected to QPCR over an extended time frame after activation of the chimera.
| Gene | -Fold Increase | Time | -Fold Increase | Time | Description |
|---|---|---|---|---|---|
| Δmax | h | Δmax | h | ||
| Rfz1 chimera | |||||
| CBP/P300-associated factor | 1.93 | 1 | 1.93 | 1 | Nuclear transcriptional coactivator protein associated factor |
| Msx-2 | 1.99 | 1 | 1.99 | 1 | Muscle segment homeobox containing gene in development |
| Follistatin | 2.46 | 1 | 2.46 | 1 | Activin-binding protein, activin antagonist |
| Lefty1 | 2.71 | 1 | 9.08 | 30 | TGF-β—related gene of early embryonic patterning |
| STAM | 1.74 | 1 | 2.57 | 45 | Signal-transducing adaptor molecule of Janus kinase |
| JAB/SOCS1 | 2.69 | 4 | 2.69 | 4 | Janus kinase-binding protein |
| Ephrin A3 | 2.13 | 4 | 2.13 | 4 | Ligand for Eph receptor tyrosine kinase |
| Msx-1 | 2.28 | 4 | 2.28 | 4 | Muscle segment homeobox containing gene in development |
| Fcer Ig | 1.86 | 4 | 2.19 | 30 | Receptor for IgA |
| Erk1 | 1.77 | 20 | 1.77 | 20 | Mitogen-activated protein kinase for cell proliferation |
| Nip-3 | 2.05 | 20 | 2.05 | 20 | E1B 19K/Bcl-2-binding protein |
| MyD118 | 2.00 | 20 | 2.36 | 45 | Growth suppression and pro-apoptotic gene |
| Rfz-2 chimera | |||||
| Msx-2 | 2.69 | 1 | 2.69 | 1 | Muscle segment homeobox containing gene in development |
| REST/NRSF | 3.20 | 1 | 3.20 | 1 | Zinc-finger gene-specific repressor element RE-1 silencing factor |
| Ubc4/5E2 | 1.81 | 2 | 1.81 | 2 | Ubiquitin-conjugating enzyme homolog of yeast Ubc4 |
| Nucleophosmin | 1.55 | 2 | 1.55 | 2 | Nucleolar phosphoprotein |
| Ephrin A3 | 1.45 | 2 | 1.70 | 4 | Ligand for Eph receptor tyrosine kinase |
| TLE/Groucho | 1.35 | 2 | 1.60 | 45 | Co-repressor mediating negative transcriptional control |
| CBP/P300-associated factor | 2.38 | 2 | 7.59 | 4 | Nuclear transcriptional coactivator protein |
| Msx-1 | 2.33 | 10 | 4.73 | 45 | Muscle segment homeobox containing gene in development |
| AA968123 | 2.17 | 20 | 2.17 | 20 | Unknown |
| Nip-3 | 2.25 | 45 | 2.25 | 45 | E1B 19K/Bcl-2-binding protein |