Effect of SB-505124 on the activities of protein kinases in vitro Protein kinases were assayed with 10 μ M SB-505124 in the presence of 0.1 mM ATP. Activities are given as the mean of duplicate determinations relative to control incubations with DMSO vehicle.
| Protein Kinase | Kinase Activity |
|---|---|
| % control | |
| AMPK | 97 |
| CDK2/cyclin A | 97 |
| Chk1 | 101 |
| CK2 | 98 |
| CSK | 100 |
| GSK3β | 97 |
| JNK1 | 103 |
| lck | 96 |
| MAPK2 | 82 |
| MAPKAP-K1β | 95 |
| MAPKAP-K2 | 81 |
| MKK1 | 94 |
| MSK1 | 98 |
| p70 S6K | 118 |
| PDK1 | 95 |
| Phos. kinase | 63 |
| PI3-kinase | 93 |
| PKA | 119 |
| PKBα | 95 |
| PKCα | 127 |
| PRAK | 96 |
| ROKα | 95 |
| p38 MAPKα | 15a |
| p38 MAPKβ | 70 |
| p38 MAPKγ | 97 |
| p38 MAPKδ | 98 |
| SGK | 77 |
AMPK, adenosine monophosphate-activated protein kinase; CDK, cyclin dependent kinase; Chk1, checkpoint kinase 1; CK2, casein kinase 2; CSK, c-src tyrosine kinase; GSK3β, glycogen synthase kinase 3β; lck, lymphocyte-specific protein kinase; MAPK2, mitogen activated protein kinase 2; MAPKAP-K1β, MAPK-activated protein kinase-1β; MAPKAP-K2, MAPK-activated protein kinase-2; MKK1, mitogen activated protein kinase kinase 1; MSK, mitogen- and stress-activated kinase; p70 S6K, 70-kDa S6 protein kinase; PDK1, phosphoinositide-dependent protein kinase1; PI3K, phosphoinositide 3-kinase; PK, protein kinase; PRAK, p38 regulated/activated protein kinase; ROK, Rho kinase; SGK, serum- and glucocorticoid-inducible kinase.
↵ a p38 MAPK α was inhibited with 10 μM SB-505124