Modification of mouse CYP2C cDNAs for expression in E. coli
Underlined bases show the NdeI restriction site and modified N-terminal sequences are shown in bold.
| cDNA | Primer Sequence for N-Terminal Modification |
|---|---|
| CYP2C50 | AAAGGATCCCATATGGCTCTGTTATTAGCAGTTTTTTTCACCCTCTCCTGTCTGTTTCTCC |
| CYP2C54 | AAAGGATCCCATATGGCTCTGTTATTAGCAGTTTTTCTCACTCTCTCCTGTCTGTTTC |
| CYP2C55 | AAAGGATCCCATATGGCTCTGTTATTAGCAGTTTTTCTCACTCTCTCCTGTCTGCTT |