Enantioselective composition of EETs produced by recombinant CYP2C enzymes
The stereochemical distribution of EETs formed by incubation of recombinant mouse CYP2C enzymes with [1-14C]AA was quantified by chiral-phase HPLC and liquid scintillation as described under Materials and Methods. Values shown are average of at least three different experiments.
| 8R,9S-EET | 8S,9R-EET | 11R,12S-EET | 11S,12R-EET | 14R,15S-EET | 14S,15R-EET | |
|---|---|---|---|---|---|---|
| % | ||||||
| CYP2C50 | 42.9 | 57.1 | 42.0 | 58.0 | 63.8 | 36.2 |
| CYP2C54 | 46.6 | 53.4 | 46.5 | 53.5 | 48.7 | 51.3 |
| CYP2C55 | 59.0 | 41.0 | 57.0 | 43.0 | 40.4 | 59.5 |