Summary of RT-qPCR data
The effect of daily oral administration of L-742694 (50 mg/kg) or DEX (50 mg/kg) for 4 days on hepatic and small intestinal gene expression in female SD rats. Results shown are fold change in mRNA level relative to vehicle control treated female SD rats, where values > 1.0 and values < 1.0 indicate induction or suppression of the gene, respectively. Values are derived from mean values of n = 3 rats ± S.E.M.; all samples were analyzed in triplicate.
| Treatment Groups | ||||
|---|---|---|---|---|
| L-742694 | DEX | |||
| Gene | Liver | Small Intestine | Liver | Small Intestine |
| CYP3A1/23 | 96.9 ± 31.4 | 0.60 ± 0.10 | 420 ± 76.6 | 0.11 ± 0.03 |
| CYP3A9 | 0.99 ± 0.20 | 0.74 ± 0.19 | 0.78 ± 0.27 | 2.68 ± 0.58 |
| CYP3A18 | 11.3 ± 4.01 | 0.42 ± 0.05 | 19.2 ± 3.20 | 0.50 ± 0.08 |
| UGT1A1 | 2.92 ± 0.40 | 0.54 ± 0.09 | 3.89 ± 0.30 | 0.29 ± 0.06 |
| UGT2B1 | 2.70 ± 0.47 | 0.23 ± 0.14 | 6.12 ± 2.01 | 0.05 ± 0.01 |
| SULT1A1 | 0.34 ± 0.05 | 0.21 ± 0.09 | 1.22 ± 0.25 | 2.48 ± 0.16 |
| Mrp2 | 0.52 ± 0.06 | 1.04 ± 0.12 | 0.28 ± 0.04 | 0.52 ± 0.11 |
| Mrp3 | 12.6 ± 0.99 | 0.45 ± 0.21 | 0.62 ± 0.16 | 0.09 ± 0.002 |
| Oatp2 | 2.68 ± 0.41 | 0.54 ± 0.26 | 3.62 ± 0.21 | 0.07 ± 0.01 |
| PXR | 1.15 ± 0.19 | 0.99 ± 0.19 | 1.25 ± 0.21 | 1.01 ± 0.28 |
| CAR | 1.26 ± 0.33 | 0.98 ± 0.20 | 1.14 ± 0.40 | 0.98 ± 0.20 |