TABLE 2

Effects of flavonoids on the cytotoxicity of mitoxantrone in MCF-7/sensitive and MCF-7 MX100 cells

The cytotoxicity of mitoxantrone in MCF-7/sensitive and MCF-7 MX100 cells was determined in the presence or absence (0.1% DMSO) of specified concentrations of flavonoids as described under Materials and Methods. For MCF-7/sensitive cells, data are the mean ± S.D. from a quadruplicate experiment; for MCF-7 MX100 cells, data are the mean ± S.D. from three separate quadruplicate experiments.

Flavonoid Concentration MCF-7/Sensitive MCF-7 MX100
10 μM 50 μM 2.5 μM 5 μM 10 μM 50 μM
Control 5.30 ± 2.22 199 ± 19.3
Apigenin 3.66 ± 0.52 3.44 ± 0.35 219 ± 10.0 10.5 ± 7.13* 1.73 ± 1.42*
Biochanin A 2.40 ± 0.27* 1.86 ± 0.35* 107 ± 17.6* 30.9 ± 5.18* 9.23 ± 2.07* 2.19 ± 1.04*
Chrysin 0.95 ± 0.46* 18.8 ± 0.06* 6.25 ± 2.13* 3.35 ± 1.70* 1.13 ± 1.11*
Genistein 6.98 ± 0.81 148 ± 23.2 29.3 ± 6.76* 2.29 ± 0.86*
Kaempferol 6.10 ± 0.96 196 ± 20.9 228 ± 13.8 3.36 ± 1.84*
Hesperetin 88.6 ± 20.8* 11.6 ± 0.83* 0.95 ± 0.19*
Naringenin 189 ± 11.6 163 ± 29.5 1.23 ± 0.16*
Silymarin 99.1 ± 50.2* 104 ± 35.5* 53.0 ± 7.27*
FTC 2.30 ± 0.29* 1.79 ± 1.52*
  • * P < 0.001.