TABLE 2

Effects of flavonoids on the cytotoxicity of mitoxantrone in MCF-7/sensitive and MCF-7 MX100 cells

The cytotoxicity of mitoxantrone in MCF-7/sensitive and MCF-7 MX100 cells was determined in the presence or absence (0.1% DMSO) of specified concentrations of flavonoids as described under Materials and Methods. For MCF-7/sensitive cells, data are the mean ± S.D. from a quadruplicate experiment; for MCF-7 MX100 cells, data are the mean ± S.D. from three separate quadruplicate experiments.

Flavonoid Concentration	MCF-7/Sensitive		MCF-7 MX100
Flavonoid Concentration	10 μM	50 μM	2.5 μM	5 μM	10 μM	50 μM
Control	5.30 ± 2.22			199 ± 19.3
Apigenin	3.66 ± 0.52	3.44 ± 0.35		219 ± 10.0	10.5 ± 7.13^*	1.73 ± 1.42^*
Biochanin A	2.40 ± 0.27^*	1.86 ± 0.35^*	107 ± 17.6^*	30.9 ± 5.18^*	9.23 ± 2.07^*	2.19 ± 1.04^*
Chrysin		0.95 ± 0.46^*	18.8 ± 0.06^*	6.25 ± 2.13^*	3.35 ± 1.70^*	1.13 ± 1.11^*
Genistein		6.98 ± 0.81		148 ± 23.2	29.3 ± 6.76^*	2.29 ± 0.86^*
Kaempferol		6.10 ± 0.96		196 ± 20.9	228 ± 13.8	3.36 ± 1.84^*
Hesperetin				88.6 ± 20.8^*	11.6 ± 0.83^*	0.95 ± 0.19^*
Naringenin				189 ± 11.6	163 ± 29.5	1.23 ± 0.16^*
Silymarin				99.1 ± 50.2^*	104 ± 35.5^*	53.0 ± 7.27^*
FTC	2.30 ± 0.29^*				1.79 ± 1.52^*

↵* P < 0.001.