Nucleotide sequences of TaqMan PCR primers and probes used in real-time RT-PCR experiments to quantify relative M1-M5 mAChR (mouse) expression levels
All probes were labeled at the 5′ end with 6-carboxyfluorescein and at the 3′ end with 6-carboxytetramethylrhodamine. Nucleotide positions refer to the receptor coding sequences
| Mouse mAChR gene | TaqMan PCR Primer and Probe Sequences | Genbank Accession No. | Nucleotide Positions |
|---|---|---|---|
| M1 | |||
| Sense | 5′-CAGAAGTGGTGATCAAGATGCCTAT-3′ | J04192 | 893-917 |
| Probe | 5′-TAGATCCTGAGGCACAGGCACCCAC-3′ | 920-944 | |
| Antisense | 5′-GAGCTTTTGGGAGGCTGCTT-3′ | 946-965 | |
| M2 | |||
| Sense | 5′-TGGAGCACAACAAGATCCAGAAT-3′ | AF264049 | 764-786 |
| Probe | 5′-CAAGGCTCTGCGGGACGGTGG-3′ | 789-809 | |
| Antisense | 5′-CCCCTGAACGCAGTTTTCA-3′ | 813-831 | |
| M3 | |||
| Sense | 5′-CCGCTCTACCTCTGTCCTTCA-3′ | AF264050 | 1352-1372 |
| Probe | 5′-AAGCCACGCTGGCTAAGAGGTTTGC-3′ | 1376-1400 | |
| Antisense | 5′-GGTGATCTGACTTCTGGTCTTGAG-3′ | 1402-1425 | |
| M4 | |||
| Sense | 5′-GTGACTGCCATCGAGATCGTAC-3′ | X63473 | 1225-1246 |
| Probe | 5′-CCAGCTGGTATGCGCCCAGCAG-3′ | 1255-1276 | |
| Antisense | 5′-CAAACTTTCGGGCCACATTG-3′ | 1278-1297 | |
| M5 | |||
| Sense | 5′-GGCCCAGAGAGAACGGAAC-3′ | AF264051 | 783-801 |
| Probe | 5′-AGGCCTCCTGGTCATCCTCCCGTA-3′ | 803-826 | |
| Antisense | 5′-TTCCCGTTGTTGAGGTGCTT-3′ | 828-847 |