Binding characteristics of WT and modified BLT1 receptors WT and mutant BLT1 receptors were transiently expressed in COS-7 cells and assayed for ligand binding as described under Materials and Methods. Dissociation constants (Kd) were calculated from competition binding curves, using GraphPad Prism version 3.0a. The results are presented as the means ± S.E.M. of at least three independent experiments, each done in triplicate.

Receptors Dissociation Constant (Kd)
Myc-BLT1 0.63 ± 0.16
BLT1-GFP 0.18 ± 0.55
BLT1-291-GFP 3.18 ± 0.89
Myc-G291stop 1.01 ± 0.15a