TABLE 2

EGFR signaling is not involved in IL-1β or PGE2 stimulated MUC5AC mucin production

Cultures were pretreated for 1 h with either media alone or media containing anti-EGFR blocking antibody (4 μg/ml) or AG-1478 (250 nM) after which time IL-1β (0 or 2.5 ng/ml) or PGE2 (150 nM) was added. After a 24-h treatment, apical secretions were collected (triplicate cultures per treatment), and the levels of MUC5AC were determined. The data are expressed as the amount of MUC5AC mucin per culture ± S.D. (arbitrary densitometric units).

Treatment EGFR inhibitors
None AG-1478 Anti-EGFR
Control 550 ± 99 517 ± 81 404 ± 115
IL-1β 1067 ± 71 1123 ± 156 1013 ± 81
PGE2 1131 ± 238 1094 ± 109 1030 ± 67