TABLE 1

Summary of growth inhibition assays for siRNA-transfected cell lines SW620, MCF-7, and HT29 cells transfected with either siGFP-22 or siDUT/230 were assayed for growth inhibition using CellTiter 96 Aqueous One Solution after treatment for 72 h with FUdR or paclitaxel. Data are presented as the mean ± S.D. of three experiments carried out in triplicate.

IC50
FUdR (72 h) Paclitaxel (72 h)
μM nM
SW620
    siGFP-22 5.50 ± 2.00 2.4 ± 2.0
    siDUT/230 0.07 ± 0.05a 2.3 ± 2.0
MCF-7
    siGFP-22 0.384 ± 0.050 1.0 ± 0.8
    siDUT/230 0.061 ± 0.030a 1.3 ± 0.7
HT29
    siGFP-22 0.0213 ± 0.010 1.9 ± 1.0
    siDUT/230 0.0102 ± 0.006 1.7 ± 1.0
  • a Significant sensitivity compared with the siGFP-22 transfected control.