Ratio of the accumulation of folate-DTPA-In111 in tumors to tissue as a function of injection frequency
Tumor-bearing mice were injected at different time intervals with a saturating dose of folate-DTPA-111In, and all mice were administered the conjugate at 40 h. Values are representative of three experiments (five mice per group). Injection times were: 1× at 40 h; 2× at 0 and 40 h; 3× at 0, 20, and 40 h; 4× at 0, 13.3, 26.6, and 40; 6× at 0, 8, 16, 24, 32, and 40 h; and 8× at 0, 5.7, 11.4, 17.1, 22.8, 28.5, 34.2, and 40 h.
| Number of Injections in 40 h | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 6 | 8 | |
| % injected dose, g of tumor:g of normal tissue | ||||||
| M109 | ||||||
| Liver | 11.4 | 11.9 | 7.33 | 5.52 | 2.97 | 3.96 |
| Kidney | 0.739 | 0.5 | 0.349 | 0.321 | 0.272 | 0.211 |
| Muscle | 12.3 | 20.1 | 12.6 | 5.96 | 5.76 | 10.6 |
| Blood | 151 | 130 | 121 | 62.6 | 31.6 | |
| Line 01 | ||||||
| Liver | 15.6 | 14.9 | 11.4 | 8.92 | 5.33 | 1.86 |
| Kidney | 0.5 | 0.57 | 0.39 | 0.33 | 0.27 | 0.12 |
| Muscle | 5.05 | 37.8 | 20.7 | 13.1 | 14.8 | 3.29 |
| Blood | 123 | 127 | 148 | 68.2 | 53.3 | 6.9 |
| L1210A | ||||||
| Liver | 7.44 | 5.75 | 4.15 | 11.7 | 7.89 | 1.94 |
| Kidney | 1.32 | 1.63 | 1.15 | 1.26 | 1.12 | 1.2 |
| Muscle | 16.7 | 10.4 | 11.1 | 20.5 | 20.5 | 29.1 |
| Blood | 380 | 156 | 111 | 90.7 | 66.9 | 52.4 |