TABLE 1

Primers Bases altered to create the Pro-to-Ala mutants are bold; bases altered to create restriction sites are italic; altered codons are underlined. The numbering of the residues assumes a 22-amino acid signal protein before the start of the mature transcript (Fig. 1).

P241A
   Forward 5′-GGCTGGGGATTTGCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGTTTATATTAC-3′
   Reverse 3′-GTAATATAAACTTCTAGCAATGGCATGTATACACGAAGGAATCAGTGCAAATCCCCAGCC-5′
P244A
   Forward 5′-GGATTTCCACTGATTGCTGCTTGTATACATGCCATTGCTAGAAGTTTATATTAC-3′
   Reverse 3′-GTAATATAAACTTCTAGCAATGGCATGTATACAAGCAGCAATCAGTGGAAATCC-5′
P275A
   Forward 5′-CCTCTACATTATCCACGGCGCAATTTGTGCTGC-3′
   Reverse 3′-GCAGCACAAATTGCGCCGTGGATAATGTAGAGG-5′
P321A
   Forward 5′-CTCTTATCTTGGTGGCATTGCTTGGCATTGAATTTGTGCTGATTCCCTGGCGACCTG-3′
   Reverse 3′-CAGGTCGCCAGGGAATCAGCACAAATTCAATGCCAAGCAATGCCACCAAGATAAGAG-5′
P331A
   Forward 5′-GAATTTGTGCTGATTGCATGGCGACCTGAAGG-3′
   Reverse 3′-CCTTCAGGTCGCCATGCAATCAGCACAAATTC-5′