TABLE 3

Expression of receptors Values are presented as mean ± S.E.M.; the number of determinations is shown in parentheses. Abmax is the relative cell surface expression of receptors as measured by detection of HA tags in an ELISA. Data normalized to WT as 100%. There is no significant difference between any value compared with WT (one-way analysis of variance followed by Dunnett's test).

Mutant Bmax Abmax
125I-CGRP [3H]BIBN4096BS
pmol/mg
WT 1.93 ± 0.40 (24) 2.99 ± 0.02 (3) 100 (3)
P241A 1.13 ± 0.43 (6) N.D. 112 ± 20 (3)
P244A 1.72 ± 0.93 (4) N.D. 131 ± 20 (3)
P275A 0.57 ± 0.14 (5) N.D. 121 ± 24 (3)
P321A 2.84 ± 0.52 (3) 2.33 ± 0.24 (3) 107 ± 24 (3)
P331A 1.75 ± 0.59 (7) N.D. 138 ± 15 (3)
  • N.D. not determined.