TABLE 5

Mutants of TM6 Values are mean ± S.E.M.; number of determinations is shown in parentheses. Emax is the maximum response expressed as a percentage of the response to the WT receptor. The pEC50 for CGRP at P321A was also significantly different from that at P321G (P < 0.05).

Mutant pEC50Emax
%
WT 8.92 ± 0.12 (7) 100
P321A 6.80 ± 024 (4)*** 98 ± 27
P321A-G324P 7.11 ± 0.22 (4)** 74 ± 13
P321A-I325P 8.86 ± 0.11 (4) 96 ± 15
P321G 7.70 ± 0.31 (4)** 84 ± 16
V320T 8.23 ± 0.36 (4)* 92 ± 25
  • Significantly different from WT at P < 0.05 (***), P < 0.01 (**), or P < 0.001 (*), as assessed by one-way ANOVA with repeated measures followed by Tukey's test.