TABLE 1

Effects of several NSAIDs on NAG-1 promoter activity

A construct, pNAG1739/LUC, was transfected into HCT-116 cells and treated with the indicated NSAIDs at different concentrations. As an internal control, pRL-null vector was cotransfected and used to adjust transfection efficiencies. The data shown is the average from three independent experiments. The estimated ED50 was calculated from the 50% induction of their maximum luciferase activity.

NSAIDs ED50
μM
Sulindac sulfide 16
Indomethacin 47
Diclofenac 57
Ibuprofen 579
Piroxicam 1260
Naproxen 1578
Sodium salicylate 4505
Aspirin 5175