Dose-response curves for current stimulation by diazepam
Cumulative concentration-response curves were carried out at different GABA concentrations. The diazepam concentrations were 1, 10, 100, and 1000 nM. Stimulation went through an optimum. At 10,000 nM, stimulation was less than at 1000 nM. Data are given as means ± S.D. from four experiments each.
| Subunit Combination | GABA | Diazepam Ka | Diazepam Emax |
|---|---|---|---|
| μM | nM | % | |
| α1 - β2 - α1/γ2 - β2 | 5 | 75 ± 15 | 255 ± 51 |
| α1 - β2 - α1/γ2 - β2Y205S | 15 | 87 ± 20 | 163 ± 22 |
| α1 - β2 - α1/γ2 - β2Y205S | 30 | 87 ± 23 | 157 ± 24 |
| α1 - β2Y205S - α1/γ2 - β2 | 15 | 47 ± 14 | 142 ± 28 |
| α1 - β2Y205S - α1/γ2 - β2 | 30 | 72 ± 10 | 106 ± 13 |
| α1 - β2Y205S - α1/γ2 - β2 | 60 | 68 ± 19 | 70 ± 9 |