TABLE 1

Primer sets for the preparation of (HUMAN)SULT2A1 5′-flanking region-luciferase reporter constructs

The underscored bases of the forward and reverse primers are KpnI and MluI sites, respectively.

Region Primer Sequences
−2595 to −1444
Forward 5′ -GCG GGT ACC ACT TGC TTG ATA AGG GAC-3′
Reverse 5′ -GCG ACG CGT TCT TCA AAG GCA AGT TGG-3′
−3688 to −2592
Forward 5′ -GCG GGT ACC GGG CAA CTG AGC ACG AGA CT-3′
Reverse 5′ -GCG ACG CGT AAG TCT GCC CTT CTC CTG AAT C-3′
−4819 to −3669
Forward 5′ -GCG GGT ACC GCC TGA GCA CCA TCT CTT CA-3′
Reverse 5′ -GCG ACG CGT AGT CTC GTG CTC AGT TGC CC-3′
−5825 to −4807
Forward 5′ -GCG GGT ACC GGC ATT TCC ATT CAA GGA CG-3′
Reverse 5′ -GCG ACG CGT ATG GTG CTC AGG CCA CGT TC-3′
−6832 to −5812
Forward 5′ -GCG GGT ACC TGT GAA ATG GAA GTA TGT GCC CAT GTG-3′
Reverse 5′ -GCG ACG CGT TGA ATG GAA ATG CCT GCT C-3′
−7626 to −6626
Forward 5′ -GCG GGT ACC GCA GGC AGA TCA CCT GAG GT-3′
Reverse 5′ -GCG ACG CGT GGC GAC AGA GTG ATA CCC TGT C-3′
−8811 to −7515
Forward 5′ -GCG GGT ACC CTG AGA CTA CAG GTG TGC AGC AC-3′
Reverse 5′ -GCG ACG CGT TAG AGC CCC CCA CCA TCA T-3′
−6042 to −5542
Forward 5′ -GCG GGT ACC GCT TGG CCT GCA ACC AAT T-3′
Reverse 5′ -GCG ACG CGT GGC CTC TGA GGA GCT GAT CT-3′
−7624 to −5817
Forward 5′ -GCG GGT ACC AGG CAG ATC ACC TGA GGT AAG GAG T-3′
Reverse 5′ -GCG ACG CGT GGA AAT GCC TGC TCT GTG AAC CTA-3′
−7624 to −5868
Forward 5′ -GCG GGT ACC CAG ATC ACC TGA GGT AAG GA-3′
Reverse 5′ -GCG ACG CGT ACA CAT TTT CCT CCC TTT G-3′
−7624 to −5905
Forward 5′ -GCG GGT ACC CAG ATC ACC TGA GGT AAG GA-3′
Reverse 5′ -GCG ACG CGT TCA CAT GCT TCC TCT GGA-3′
−7624 to −5955
Forward 5′ -GCG GGT ACC CAG ATC ACC TGA GGT AAG GA-3′
Reverse 5′ -GCG ACG CGT CTT ATC TTT CTG TGA TTG-3′
−7624 to −5995
Forward 5′ -GCG GGT ACC CAG ATC ACC TGA GGT AAG GA-3′
Reverse 5′ -GCG ACG CGT GCA TAA AGT GTA ACA TCA-3′