Rolipram inhibition | Unlike 4A4, particulate and soluble forms of 4A11 show no difference in sensitivity to inhibition by rolipram |
Long-term rolipram treatment | Unlike 4A4, chronic treatment of cells with rolipram does not cause redistribution of 4A11 within cells |
Ro 20-1724 | 4A11 and 4A10 are more potently inhibited than 4A4 |
Roflumilast | Unlike 4A4, soluble and particulate 4A11 forms show similar sensitivity to inhibition by roflumilast |
Denbufylline | 4A11 is more sensitive to inhibition than 4A4 or 4A10 |
Ariflo (cilomilast) | 4A10 is less sensitive to inhibition than 4A4 or 4A11 |
Distribution | Unlike 4A4 and 4A11, 4A11 is found in plasma membrane ruffles |
LYN (SH3 domain) | 4A11 and 4A10 show a lower propensity of associating with LYN through its SH3 domain than does 4A4 |
Thermal stability of soluble 4A11 | Soluble 4A11 is dramatically more thermolabile than the soluble form of other PDE4A long isoforms |
Thermal stability of particulate 4A11 | Particulate 4A11 is more thermolabile than 4A4 but similarly thermolabile to 4A10 |
Comparative thermostability of soluble and particulate forms | Although particulate 4A11 is more thermostable than cytosolic 4A11, the converse is true for both 4A10 and 4A4 |
Caspase-3 | Only PDE4A4 is cleaved by caspase-3 |
β—arrestin2 | All three isoforms can interact with β—arrestin2 |
PKA phosphorylation | Similar to other PDE4A long isoforms |
Km cAMP | Similar to other PDE4A long isoforms |
Vmax cAMP hydrolysis | Similar to other PDE4A long isoforms |