Oligonucleotides used for PCR-based site-directed deletions
| Plasmid | 5′-NT | Primer Sequence |
|---|---|---|
| Deletion C/EBPβ Δ1 | -5885 | 5′-CACATCTCATGATGATCTTCAT-3′ FP |
| -5871 | 5′-AAAACGTCAGCACAACCTAC-3′ RP | |
| Deletion C/EBPβ Δ2 | -5800 | 5′-CTCACATTCTCTGATTTCTCTTT-3′ FP |
| -5786 | 5′-AGAAGAGCACGTGGAAGG-3′ RP | |
| Deletion C/EBPβ Δ3 | -5694 | 5′-AACATTTGCCTTTCTGCTC-3′ FP |
| -5680 | 5′-CATGTCACAATCAGAATCATTT-3′ RP |
NT, nucleotide; FP, forward primer; RP, reverse primer.