TABLE 1

EC50 values for opioid agonists, DAMGO, DPDPE, and U69593 obtained from [35S]GTPγS binding dose-response curves performed in wild-type or GFP/Rluc-fused opioid receptor transfected HEK cell membranes

Data presented as mean ± S.E.; n = 3.

Receptor DAMGO DPDPE U69593
hMOR 27 ± 15 N.D. N.D.
hMOR-GFP 12 ± 11 N.D. N.D.
hMOR-Rluc 18 ± 14 N.D. N.D.
mDOR N.D. 2.6 ± 1.2 N.D.
mDOR-GFP N.D. 1.7 ± 1.3 N.D.
mDOR-Rluc N.D. 4.4 ± 1.3 N.D.
hKOR N.D. N.D. 3.6 ± 2.1
hKOR-GFP N.D. N.D. 1.2 ± 1.5
hKOR-Rluc N.D. N.D. 1.8 ± 1.8
  • DPDPE, [d-Pen2,d-Pen5]-enkephalin; N.D., not determined.