Effects of gemcitabine and pemetrexed on cell cycle and induction of apoptosis of NSCLC cell lines
Cancer cells were exposed to IC50 values of drugs alone and in combination at fixed 1:2, 1:6, and 1:3 (gemcitabine/pemetrexed) concentration ratios in A549, Calu-1, and Calu-6 cells, respectively. Values are presented as the mean percentage of the total number of cells examined in three independent experiments.
| Cell Treatment | G1 | S | G2 | Sub-G1 |
|---|---|---|---|---|
| % | ||||
| A549 | ||||
| Control | 90.71 | 5.95 | 3.34 | 1.8 |
| Gemcitabine | 68.53 | 18.02 | 13.46 | 12.9 |
| Pemetrexed | 58.83 | 32.48 | 8.70 | 6.9 |
| Gemcitabine→pemetrexed | 49.59 | 40.89 | 9.51 | 21.0 |
| Pemetrexed→gemcitabine | 45.25 | 39.68 | 15.07 | 25.1 |
| Calu-1 | ||||
| Control | 59.58 | 25.68 | 14.74 | 2.1 |
| Gemcitabine | 57.65 | 30.70 | 11.66 | 9.3 |
| Pemetrexed | 54.22 | 38.42 | 2.66 | 5.4 |
| Gemcitabine→pemetrexed | 39.00 | 35.57 | 25.43 | 10.6 |
| Pemetrexed→gemcitabine | 45.26 | 36.42 | 18.32 | 12.0 |
| Calu-6 | ||||
| Control | 50.46 | 35.01 | 14.54 | 5.3 |
| Gemcitabine | 64.10 | 31.62 | 4.27 | 9.4 |
| Pemetrexed | 74.20 | 25.75 | 5.95 | 6.2 |
| Gemcitabine→pemetrexed | 20.49 | 54.03 | 25.48 | 17.1 |
| Pemetrexed→gemcitabine | 27.97 | 44.85 | 27.18 | 18.8 |