TABLE 6

Effects of pemetrexed, at IC50 level, on dCK and hENT1 gene expression of NSCLC cell lines 6,12, 24, and 48 h after the end of treatment

The amount of target gene, normalized to GAPDH and relative to the calibrator (untreated control cells), is given as 2-ΔΔCT.

Times dCK hENT1
A549 Cells
    6 h 1.24 1.11
    12 h 1.52 1.78
    24 h 1.92 1.52
    48 h 1.97 1.29
Calu-1 Cells
    6 h 0.76 1.31
    12 h 0.95 1.34
    24 h 0.87 1.39
    48 h 1.01 1.71
Calu-6 Cells
    6 h 0.51 1.18
    12 h 1.77 1.51
    24 h 1.41 1.51
    48 h 2.12 0.76