pcDNA3.1(+) clones The parent plasmid contains a CMV promoter that yields robust expression of cloned sequences when transfected into mammalian cells. This plasmid is referred to in the text as “3.1”.
| Name | PCR/Mutagenesis Primers | Amino Acids | Restriction Sites |
|---|---|---|---|
| HA-CAR3 | FP: GGAATTCGCCACCATGGC ATACCCATACGATGTTCCAGA TTACGCTATGGCCAGTAGGGA AGATGAGC | N-terminal HA-Tag-1–353 | EcoR1/Xba1 |
| RP: BGH | |||
| CAR3-LBD | FP: GATCGAATTCGCCACCAT GGTACTGTCGGCAGAAGCCC | 80–352 | EcoR1/Xba1 |
| RP: BGH | |||
| CAR3-ΔAF2 | FP: GATCGAATTCGTCATGGCCAGTAGGGAAGATGAG | 1–345 | EcoR1/Xba1 |
| RP: GTCTAGACTACATCA TGGCAGACAGGCCCTG | |||
| RXRα | FP: GATCGAATTCGCCGCCAT GGACACCAAACATTTCCTG | 1–462 | EcoR1/Xba1 |
| RP: GATCTCTAGACTAAGTCATTT GGTGCGGCGC | |||
| RXRα-ΔAF1 | FP: GGAATTCGCCACCATGGC CCACCCCTCAGGAAACATG | 123–462 | EcoR1/Xba1 |
| RP: GATCTCTAGACTAAGTCATTT GGTGCGGCGC | |||
| RXRα- LBD | FP: GGAATTCGCCGCCATGGG CATGAAGCGGGAAG | 198–462 | EcoR1/Xba1 |
| RP: GATCTCTAGACTAAGTCATTT GGTGCGGCGC | |||
| RXRα-ΔAF2 | FP: GATCGAATTCGCCGCCAT GGA CACCAAACATTTCCTG | 1–449 | EcoR1/Xba1 |
| RP: GATCTCTAGATTAGGTGTCAA TGGGTGTGTCCC | |||
| RXRα-Y397A | FP: GATCGAATTCGCCGCCAT GGACACCAAACATTTCCTG | 1–462 | EcoR1/Xba1 |
| RP: GATCTCTAGACTAAGTCATTTGGTGCGGCGC | |||
| mtFP: GGAGAAGGTCGCTGCGTCCTTGGAGGCCTACTGCAAG | |||
| mtRP: CTTGCAGTAGGCCTCCAAGGACGCAGCGACCTTCTCC |
FP, forward primer; RP, reverse primer.