Antibody | Positive Tissues | Negative Control Tissues | ||||
---|---|---|---|---|---|---|
α2 | α2β4 cells | 85 ± 5% | n = 4 | Pineal gland (α3β4)a | <1% | n = 3 |
Cerebral cortex (α4β2)b | <1% | n = 3 | ||||
α3β4 cells | <1% | n = 3 | ||||
α3 | Pineal gland | 99 ± 1% | n = 6 | α2β4 cells | <1% | n = 3 |
Sup cervical ganglia | 92 ± 2% | n = 3 | α4β2 cells | <1% | n = 3 | |
α4 | Cerebral cortex | 83 ± 5% | n = 10 | Pineal gland | <1% | n = 3 |
α3β4 cells | <1% | n = 3 | ||||
α6 | Superior colliculus | 25 ± 1% | n = 3 | Cerebral cortex | <2% | n = 4 |
Pineal gland | <1% | n = 5 | ||||
α3β4 cells | <2% | n = 3 | ||||
β2 | Cerebral cortex | 94 ± 7% | n = 14 | Pineal gland | <2% | n = 4 |
α4β2 cells | 99 ± 1% | n = 2 | α2β4 cells | <2% | n = 3 | |
α3β4 cells | <2% | n = 3 | ||||
β4 | Pineal gland | 100 ± 3% | n = 6 | Cerebral cortex | <2% | n = 4 |
α4β2 cells | <1% | n = 3 | ||||
β3 | Striatum | 8 ± 1% | n = 4 | Pineal gland | <1% | n = 3 |
Superior colliculus | 9 ± 1% | n = 7 | Cerebral cortex | <2% | n = 3 |
↵ a Virtually all of the nAChRs in the pineal gland are an α3β4 subtype (Hernandez et al., 2004)
↵ b The nAChRs in the cerebral cortex are predominantly an α4β2 subtype (Whiting et al. 1987; Flores et al., 1992)