TABLE 1

Saturation ligand binding analyses for [³H]nemonapride, [³H]raclopride, and [³H]spiperone binding to D₂ dopamine receptors expressed in Sf9 cells Saturation binding studies were performed as described under Materials and Methods section with [³H]nemonapride, [³H]raclopride, and [³H]spiperone using different preparations of Sf9 cell membranes expressing the D₂ dopamine receptor, under control conditions or with 100 mM sodium ions. Experiments with preparation 3 included 0.1% bovine serum albumin as described under Materials and Methods. All saturation analyses fitted best to one-binding site models from which B_max (pmol/mg) and pK_d values were determined and are expressed as mean ± S.E.M. from three or more experiments.