TABLE 1

Inhibitory effect of plasmid driven HIF-1-α-no-TAD expression on HRE reporter output

-Fold inhibition was calculated by dividing the luciferase activity obtained upon transfection of reporter alone with the activity obtained when co-transfected with HIF-1α-no-TAD.

Cell Line Tumor Type LDH PGK CA-9 VEGF
Air Anoxia Air Anoxia Air Anoxia Air Anoxia
T47D Breast 0.3 6.5 0.8 5.2 0.8 5.4 1.3 0.5
HT1080 Fibrosarcoma 4.7 5.2 1.7 6.0 2.3 5.4 0.4 1.5
DU145 Prostate 7.1 3.0
HCT116 Colon 0.4 181.5
U87 Glioma 16.2 14.3
HT29 Colon 1.5 33.7