TABLE 2

Effect of TSO administration on mRNA expression in livers from wild-type and CAR-null mice

Total RNA was isolated from livers of wild-type and CAR-null mice after 4 days of TSO administration (200 mg/kg, i.p.) and analyzed by the bDNA signal amplification assay for Cyp2b10. Nqo1, Eh, Ho-1, Ugt1a1, Ugt1a6, Ugt2b5, Mrp2, Mrp3, and Mrp4 mRNA content. The data is presented as mean RLU ± SEM. (n = 3-4 animals).

Wild-Type CAR-Null
Vehicle TSO Vehicle TSO
Cyp2b10 14.5 ± 1.0 426.5 ± 8.0* 1.6 ± 0.2# 54.6 ± 1.3*†
Nqo1 10.4 ± 2.1 53.0 ± 10.4* 9.1 ± 0.9 39.5 ± 6.1*
Eh 767.8 ± 135.1 1701.3 ± 172.0* 533 ± 39.7 888.6 ± 97.6*†
Ho-1 66.4 ± 6.6 62.2 ± 9.3 27 ± 5.5# 23.9 ± 6.4#
Ugt1a1 166.5 ± 20.0 187.2 ± 10.6 58.1 ± 11.8# 61.1 ± 3.0#
Ugt1a6 143.3 ± 33.8 175.2 ± 3.5 68.3 ± 10.9# 84.7 ± 4.0#
Ugt2b5 920.7 ± 195.6 1479.7 ± 97.3* 642.9 ± 75.0 921.5 ± 84.1*†
Mrp2 514.2 ± 122.6 588.6 ± 38.5 311.2 ± 18.6# 439.1 ± 43.7*
Mrp3 149.0 ± 34.5 339.4 ± 43.7* 93.1 ± 10.0 170.0 ± 15.2*
Mrp4 3.7 ± 0.7 5.8 ± 0.7 2.4 ± 0.2 4.2 ± 0.9

  • ↵ * Statistical difference (P < 0.05) between control and TSO-treated groups.

  • ↵# Statistical difference (P < 0.05) between WT and CAR-null control groups.

  • ↵† Statistical difference (P < 0.05) between WT and CAR-null mice treated with TSO.