Inhibition of Clonogenicity LC50 | |||||
---|---|---|---|---|---|
Cell Line with Transfection | l-Configuration | d-Configuration | |||
Dox (100 ng/ml) | l-OddC | l-Fd4C | dFdC | AraC | |
nM | μM | nM | nM | ||
Con ShRNA | − | 158 ± 18 | 5.4 ± 1.8 | 2.4 ± 0.5 | 16.5 ± 3.5 |
+ | 163 ± 19 | 5.9 ± 2.3 | 2.5 ± 0.7 | 15 ± 4.5 | |
shRNA | − | 119 ± 20 | 6.1 ± 1.1 | 3.2 ± 1.1 | 20.7 ± 4.7 |
+ | 56 ± 5 | 3.2 ± 0.9 | 3.4 ± 1.2 | 21.7 ± 5.6 | |
Con pcDNA5 | − | 125 ± 35 | 5.5 ± 0.7 | 2.5 ± 0.6 | 12.2 ± 3.8 |
+ | 128 ± 25 | 6.5 ± 0.6 | 2.6 ± 0.8 | 13 ± 2.8 | |
Wt-APE-1 | − | 108 ± 25 | 4.3 ± 1.2 | 2.5 ± 0.4 | 16.5 ± 6.5 |
+ | 238 ± 53 | 9.2 ± 2.7 | 2.6 ± 0.3 | 17.5 ± 2.5 | |
C65A-APE-1 | − | 158 ± 25 | 5.6 ± 1.4 | 2.5 ± 0.1 | 18.5 ± 3.5 |
+ | 263 ± 18 | 10.9 ± 2.3 | 2.7 ± 0.2 | 21 ± 1.4 | |
E96A-APE-1 | − | 123 ± 4 | 4.5 ± 0.4 | 2.5 ± 0.1 | 14 ± 5 |
+ | 130 ± 7 | 4.3 ± 0.5 | 2.6 ± 0.1 | 15 ± 6 | |
E96Q-APE-1 | − | 140 ± 27 | 4.9 ± 0.5 | 2.4 ± 0.3 | 19 ± 3 |
+ | 133 ± 19 | 5.2 ± 0.3 | 2.2 ± 0.2 | 17 ± 5 |